Decellularized Bovine Skeletal Muscle Scaffolds: Structural Characterization and Preliminary Cytocompatibility Evaluation.

Skeletal muscle degeneration is responsible for major mobility complications, and this muscle type has little regenerative capacity. Several biomaterials have been proposed to induce muscle regeneration and function restoration. Decellularized scaffolds present biological properties that allow efficient cell culture, providing a suitable microenvironment for artificial construct development and being an alternative for in vitro muscle culture. For translational purposes, biomaterials derived from large animals are an interesting and unexplored source for muscle scaffold production. Therefore, this study aimed to produce and characterize bovine muscle scaffolds to be applied to muscle cell 3D cultures. Bovine muscle fragments were immersed in decellularizing solutions for 7 days. Decellularization efficiency, structure, composition, and three-dimensionality were evaluated. Bovine fetal myoblasts were cultured on the scaffolds for 10 days to attest cytocompatibility. Decellularization was confirmed by DAPI staining and DNA quantification. Histological and immunohistochemical analysis attested to the preservation of main ECM components. SEM analysis demonstrated that the 3D structure was maintained. In addition, after 10 days, fetal myoblasts were able to adhere and proliferate on the scaffolds, attesting to their cytocompatibility. These data, even preliminary, infer that generated bovine muscular scaffolds were well structured, with preserved composition and allowed cell culture. This study demonstrated that biomaterials derived from bovine muscle could be used in tissue engineering.

Grain Products: Traditional and Innovative Technologies.

The current knowledge regarding various cereals, their composition, and their processing methods is extensive [...].

Chemical profiles and bioactivities of polyphenolic extracts of Lavandula stoechas L., Artemisia dracunculus L. and Ocimum basilicum L.

This study assessed the chemical profiles and bioactivities of the infusions, decoctions and hydroethanolic extracts of tarragon, basil and French lavender. The extracts were chemically characterised (HPLC-DAD-ESI/MS) and their bioactivities were evaluated in vitro. All extracts revealed antimicrobial, antifungal and antioxidant properties. French lavender extracts showed higher total phenolic content, regardless of the extraction method used, and antioxidant and antitumour capacities, but no anti-inflammatory action. All basil and two of the tarragon extracts revealed anti-inflammatory power. Thus, tarragon, basil and French lavender extracts may be considered for inclusion in foods, as preservatives or functional ingredients. Nonetheless, further studies must be conducted to evaluate the pharmacokinetic parameters of the bioactive compounds.

Nutritional profiling, fiber content and in vitro bioactivities of wheat-based biscuits formulated with novel ingredients.

This study evaluated the nutritional profile and fiber content of innovative formulations of wheat-based biscuits enriched with chia seeds, carob flour and coconut sugar. The in vitro antioxidant, cytotoxic, anti-inflammatory and antimicrobial activities were also investigated to understand the potential health advantages of the incorporation of these new ingredients. The novel biscuits demonstrated significant improvements in protein and mineral content, with increases of 50% and 100% in chia biscuits, and up to 20% and 40% in carob biscuits, respectively. Fiber also notably increased, particularly in samples containing 10% carob flour, which increased four times as compared to wheat-based samples. The new ingredients exhibited antibacterial and antifungal activity, particularly against Yersinia enterocolitica (minimum inhibitory concentration 1.25 mg mL-1 in coconut sugar) and Aspergillus fumigatus (minimum inhibitory concentration/minimum fungicidal concentrations 2.5/5 mg mL-1 in chia seeds). However, the final biscuits only displayed antifungal properties. Carob flour and chia seeds had a remarkably high capacity to inhibit the formation of TBARS and promoted greater antioxidant activity in biscuit formulations, with EC50 values decreasing from 23.25 mg mL-1 (control) to 4.54 mg mL-1 (15% defatted ground chia seeds) and 1.19 mg mL-1 (10% carob flour). Only chia seeds exhibited cellular antioxidant, anti-inflammatory and cytotoxic activity, attributes that were lost when seeds were added into the biscuits. These findings highlight the potential health benefits of these ingredients, particularly when incorporated in new wheat-based formulations.

Polyphenol Composition by HPLC-DAD-(ESI-)MS/MS and Bioactivities of Extracts from Grape Agri-Food Wastes.

BACKGROUND: Grape agri-food wastes, such as skin, seeds, and other discarded by-products, contain phytochemical compounds that offer potential health benefits. METHODS: This study aimed to investigate the polyphenol composition and bioactivities of different extracts obtained from grape marc and seeds, with the goal of exploring their potential for application as natural food additives. RESULTS: Regardless of the extraction method used (dynamic maceration, ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE)), all extracts exhibited relatively high concentrations of phenolic compounds. The chemical characterization of the extracts revealed the presence of specific compounds and chemical groups associated with each extraction methodology. Moreover, the extracts displayed satisfactory antioxidant activities, especially in inhibiting lipoperoxidation as assessed by the TBARS assay. Additionally, the extracts demonstrated effective inhibition against different strains of bacteria and fungi known as food contaminants. Taken together, these findings indicate that those extracts have the potential to be tested as natural antioxidants and preservatives with sustainable origins in food and beverage systems. Among the extraction methods evaluated, traditional maceration and UAE provided extracts with the highest antioxidant and antimicrobial activities. CONCLUSIONS: Our results suggest the opportunity to explore grape marc and seeds discarded by the winery industry in Portugal as natural sources of bioactive compounds, which could be employed as functional food ingredients or technological additives. The valorization of grape biowastes offers a promising strategy to reduce waste and harness their potential health benefits.

Use of Bio-Waste of Ilex paraguariensis A. St. Hil. (Yerba mate) to Obtain an Extract Rich in Phenolic Compounds with Preservative Potential.

In this work, a comparison between the extracts of dehydrated yerba mate (Ilex paraguariensis) and bio-waste of yerba mate leaves from the Brazilian industry was made. The incorporation of the functional extract as a preservative/functional ingredient in a pastry product (pancakes) was tested. The individual profile of phenolic compounds was determined by HPLC-DAD-ESI/MS, and the bioactive potential was assessed using in vitro assays for antioxidant, anti-inflammatory, antimicrobial, and cytotoxic activities. The yerba mate extracts revealed a high antimicrobial potential against the tested strains and a very promising antioxidant and anti-inflammatory action. Additionally, revealed a cytotoxic capacity for MCF-7, CaCo and AGS tumor cell-lines. In the three types of pancakes, after 3 days of storage, the chemical and nutritional characteristics remain unchanged, proving the preservative efficiency of the extract. This study showed the benefits of the use bio-waste from agro-industrial sector, focusing on sustainable production and the development of circular economy.

Morphofunctional Improvement of the Facial Nerve and Muscles with Repair Using Heterologous Fibrin Biopolymer and Photobiomodulation.

Peripheral nerve injuries impair the patient's functional capacity, including those occurring in the facial nerve, which require effective medical treatment. Thus, we investigated the use of heterologous fibrin biopolymer (HFB) in the repair of the buccal branch of the facial nerve (BBFN) associated with photobiomodulation (PBM), using a low-level laser (LLLT), analyzing the effects on axons, muscles facials, and functional recovery. This experimental study used twenty-one rats randomly divided into three groups of seven animals, using the BBFN bilaterally (the left nerve was used for LLLT): Control group-normal and laser (CGn and CGl); Denervated group-normal and laser (DGn and DGl); Experimental Repair Group-normal and laser (ERGn and ERGl). The photobiomodulation protocol began in the immediate postoperative period and continued for 5 weeks with a weekly application. After 6 weeks of the experiment, the BBFN and the perioral muscles were collected. A significant difference (p < 0.05) was observed in nerve fiber diameter (7.10 ± 0.25 µm and 8.00 ± 0.36 µm, respectively) and axon diameter (3.31 ± 0.19 µm and 4.07 ± 0.27 µm, respectively) between ERGn and ERGl. In the area of muscle fibers, ERGl was similar to GC. In the functional analysis, the ERGn and the ERGI (4.38 ± 0.10) and the ERGI (4.56 ± 0.11) showed parameters of normality. We show that HFB and PBM had positive effects on the morphological and functional stimulation of the buccal branch of the facial nerve, being an alternative and favorable for the regeneration of severe injuries.

Phytochemical Composition and Bioactive Potential of Melissa officinalis L., Salvia officinalis L. and Mentha spicata L. Extracts.

Plants are rich in bioactive phytochemicals that often display medicinal properties. These can play an important role in the production of health-promoting food additives and the replacement of artificial ones. In this sense, this study aimed to characterise the polyphenolic profile and bioactive properties of the decoctions, infusions and hydroethanolic extracts of three plants: lemon balm (Melissa officinalis L.), sage (Salvia officinalis L.) and spearmint (Mentha spicata L.). Total phenolic content ranged from 38.79 mg/g extract to 84.51 mg/g extract, depending on the extract. The main phenolic compound detected in all cases was rosmarinic acid. The results highlighted that some of these extracts may have the ability to prevent food spoilage (due to antibacterial and antifungal effects) and promote health benefits (due to anti-inflammatory and antioxidant capacities) while not displaying toxicity against healthy cells. Furthermore, although no anti-inflammatory capacity was observed from sage extracts, these stood out for often displaying the best outcomes in terms of other bioactivities. Overall, the results of our research provide insight into the potential of plant extracts as a source of active phytochemicals and as natural food additives. They also support the current trends in the food industry of replacing synthetic additives and developing foods with added beneficial health effects beyond basic nutrition.

First official record of Aedes (Stegomyia) albopictus (Diptera: Culicidae) in the Acre State, Northern Brazil.

Aedes (Stegomyia) albopictus (Skuse, 1854) was reported in Brazil for the first time in 1986 and has shown marked expansion throughout the Brazilian territory. During a routine activity to control dengue fever conducted by the Division of Entomology of the Municipal Health Department in Rio Branco city, adults and immatures of Culicidae were collected in a peri-urban area. The identified Culicidae forms indicated that they belonged to the species Ae. albopictus. This is the first official record of the presence of Ae. albopictus in the Acre State, confirming its current presence in all Brazilian states.

Relato de experiência: grupo de saúde mental para adolescentes em tempos de pandemia da COVID-19 / Experience report: mental health group for adolescents in times of the COVID-19 pandemic / Informe de experiencia: grupo de salud mental para adolescentes en tiempos de la pandemia de COVID-19

A pandemia da COVID-19 introduziu diversos estressores e descortinou questões relacionadas à existência humana, atingindo significativamente a saúde mental dos indivíduos. A Estratégia Saúde da Família (ESF) é um cenário privilegiado para ações em saúde mental na Atenção Básica (AB), e, com isso, surgiu a proposta da criação do grupo de compartilhamento para adolescentes em sofrimento psíquico. OBJETIVOS: Oferecer escuta e suporte emocional ao público adolescente do território de abrangência de uma Unidade de Saúde da Família (USF), e favorecer a criação de estratégias de enfrentamento. METODOLOGIA: Trata-se de uma pesquisa-ação de abordagem qualitativa, do tipo relato de experiência, onde buscou-se refletir acerca da vivência grupal no decorrer de quatro meses em uma Residência Multiprofissional em Saúde. Como embasamento teórico-metodológico, utilizou-se as propostas da logoterapia e Análise Existencial de Viktor Emil Frankl. RESULTADOS: Foram desenvolvidas ações que propiciaram o desenvolvimento de aspectos como percepção de si, autenticidade e responsabilidade. Durante o processo, foi possível perceber a aproximação da equipe com a realidade desse público e a apropriação na condução das ações em saúde mental. CONCLUSÃO: O presente trabalho permite refletir sobre a importância das ações em saúde mental junto ao público juvenil, visto que o aumento da sensação de sentido e de valor da vida configura-se como fator importante de prevenção de crises e agravos psicológicos, especialmente em contexto da pandemia de COVID-19.

The COVID-19 pandemic brought in several stressor points and uncovered aspects related to human existence, deeply affecting the subjects' mental health. The Family Health Strategy is a privileged arena for the consolidation to mental health care in the Basic Attention. Taking that into account, the proposal for creating a group for sharing among teenagers with mental suffering came out. OBJECTIVES: To offer qualified listening and emotional support to the teenagers living in a territory covered by a Unit of Health Family as well as to promote the development of strategies of confrontation. METHODOLOGY: This is a qualitative research-action, an experience report, that aimed to reflect about the group perception for four months during the period of the Health Multidisciplinary Residence. It was used as theoreticalmethodological fundamentals of Logotherapy and Existential Analysis, both theories developed by Viktor Emil Frankl. RESULTS: It was built up actions that provided the development of aspects such as self-perception, authenticity and responsibility. During the process, it was possible to realize the approximation of the staff with the situation of the teenagers as well as the possibility of the staff to lead the actions aiming at mental health. CONCLUSION: This work allowed us to reflect on the meaning of the actions to develop mental health in teenagers since the increase of the worth and meaning of life denoted an important aspect for preventing psychological crisis and damages, mainly in the COVID-19 pandemic context.

La pandemia de COVID-19 introdujo varios estresores y destapó cuestiones relacionadas con la existencia humana, afectando significativamente la salud mental de las personas. La Estrategia Salud de la Familia (ESF) es un escenario privilegiado para las acciones en salud mental en la Atención Básica (AB), y con eso surgió la propuesta de crear un grupo de convivencia para adolescentes en sufrimiento psíquico. OBJETIVOS: Ofrecer escucha y apoyo emocional al público adolescente en el territorio de actuación de una Unidad de Salud de la Familia (USF), y favorecer la creación de estrategias de enfrentamiento. METODOLOGÍA: Se trata de una investigación-acción con abordaje cualitativo, del tipo relato de experiencia, donde se buscó reflexionar sobre la experiencia grupal a lo largo de cuatro meses en una Residencia Multidisciplinaria en Salud. Como base teórico-metodológica se utilizaron las propuestas de logoterapia y Análisis Existencial de Viktor Emil Frankl. RESULTADOS: Se desarrollaron acciones que favorecieron el desarrollo de aspectos como la autopercepción, la autenticidad y la responsabilidad. Durante el proceso, fue posible percibir la aproximación del equipo con la realidad de este público y la apropiación en la conducción de acciones en salud mental. CONCLUSIÓN: El presente trabajo permite reflexionar sobre la importancia de las acciones en salud mental con el público joven, ya que el aumento del sentido y valor de la vida es un factor importante en la prevención de crisis y trastornos psicológicos, especialmente en un contexto de la pandemia del COVID-19.

Influence of the Neuroprotective Properties of Quercetin on Regeneration and Functional Recovery of the Nervous System.

Quercetin is a dietary flavonoid present in vegetables, fruits, and beverages, such as onions, apples, broccoli, berries, citrus fruits, tea, and red wine. Flavonoids have antioxidant and anti-inflammatory effects, acting in the prevention of several diseases. Quercetin also has neuroprotective properties and may exert a beneficial effect on nervous tissue. In this literature review, we compiled in vivo studies that investigated the effect of quercetin on regeneration and functional recovery of the central and peripheral nervous system. In spinal cord injuries (SCI), quercetin administration favored axonal regeneration and recovery of locomotor capacity, significantly improving electrophysiological parameters. Quercetin reduced edema, neutrophil infiltration, cystic cavity formation, reactive oxygen species production, and pro-inflammatory cytokine synthesis, while favoring an increase in levels of anti-inflammatory cytokines, minimizing tissue damage in SCI models. In addition, the association of quercetin with mesenchymal stromal cells transplantation had a synergistic neuroprotective effect on spinal cord injury. Similarly, in sciatic nerve injuries, quercetin favored and accelerated sensory and motor recovery, reducing muscle atrophy. In these models, quercetin significantly inhibited oxidative stress and cell apoptosis, favoring Schwann cell proliferation and nerve fiber remyelination, thus promoting a significant increase in the number and diameter of myelinated fibers. Although there is still a lack of clinical research, in vivo studies have shown that quercetin contributed to the recovery of neurological functions, exerting a beneficial effect on the regeneration of the central and peripheral nervous system.

First official record of Aedes (Stegomyia) albopictus (Diptera: Culicidae) in the Acre State, Northern Brazil

ABSTRACT Aedes (Stegomyia) albopictus (Skuse, 1854) was reported in Brazil for the first time in 1986 and has shown marked expansion throughout the Brazilian territory. During a routine activity to control dengue fever conducted by the Division of Entomology of the Municipal Health Department in Rio Branco city, adults and immatures of Culicidae were collected in a peri-urban area. The identified Culicidae forms indicated that they belonged to the species Ae. albopictus. This is the first official record of the presence of Ae. albopictus in the Acre State, confirming its current presence in all Brazilian states.

Immunological dimensions of neuroinflammation and microglial activation: exploring innovative immunomodulatory approaches to mitigate neuroinflammatory progression.

The increasing life expectancy has led to a higher incidence of age-related neurodegenerative conditions. Within this framework, neuroinflammation emerges as a significant contributing factor. It involves the activation of microglia and astrocytes, leading to the release of pro-inflammatory cytokines and chemokines and the infiltration of peripheral leukocytes into the central nervous system (CNS). These instances result in neuronal damage and neurodegeneration through activated nucleotide-binding domain and leucine-rich repeat containing (NLR) family pyrin domain containing protein 3 (NLRP3) and nuclear factor kappa B (NF-kB) pathways and decreased nuclear factor erythroid 2-related factor 2 (Nrf2) activity. Due to limited effectiveness regarding the inhibition of neuroinflammatory targets using conventional drugs, there is challenging growth in the search for innovative therapies for alleviating neuroinflammation in CNS diseases or even before their onset. Our results indicate that interventions focusing on Interleukin-Driven Immunomodulation, Chemokine (CXC) Receptor Signaling and Expression, Cold Exposure, and Fibrin-Targeted strategies significantly promise to mitigate neuroinflammatory processes. These approaches demonstrate potential anti-neuroinflammatory effects, addressing conditions such as Multiple Sclerosis, Experimental autoimmune encephalomyelitis, Parkinson's Disease, and Alzheimer's Disease. While the findings are promising, immunomodulatory therapies often face limitations due to Immune-Related Adverse Events. Therefore, the conduction of randomized clinical trials in this matter is mandatory, and will pave the way for a promising future in the development of new medicines with specific therapeutic targets.

Comparative study on the phenolic composition and in vitro bioactivity of medicinal and aromatic plants from the Lamiaceae family.

Medicinal and aromatic plants (MAP) have been described as a source of phenolic compounds with potential as antioxidant, antiproliferative and antimicrobial agents. MAP from the Lamiaceae family (Origanum vulgare L., Thymus vulgaris L., Ocimum basilicum L., Salvia officinalis L., Melissa officinalis L., and MatricariachamomillaL.) were selected to perform a phytochemical and biological screening for their further exploitation as natural bioactive ingredients. The total content of phenolic compounds varied from 184.02 mg/g extract in M. officinalis to 17.97 mg/g extract in M. chamomilla. Caffeic and rosmarinic acids were the main phenolic acids found in the respective hydroalcoholic extracts. The extracts showed a promising antioxidant activity in vitro, being related the phenolic compositions of the extracts, furthermore, all extracts being able to combat lipid peroxidation in TBARS assays with an IC50 under 26 µg/mL, moreover all the plant extract has prevented the oxidative haemolysis in OxHLIA assays at concentrations below 67 µg/mL in a Δt 60 min and under 118 µg/mL for a Δt 120 min. Regarding to the bactericidal and fungicidal action the plant extracts were able to inhibit growth against bacteria associated with food hazards, such as Salmonella typhimurium (MIC < 1) and Listeria monocytogenes (MIC < 1), regarding to fungicidal activity it can be highlighted the MIC values under to 0.25 for Aspergillus versicolor and Trichoderma viride. Overall, the selected Lamiaceae plants stood out as a source of active phytochemicals that can be used by different industries, such as food and cosmetics.

From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants.

Several scientific studies have been proving the bioactive effects of many aromatic and medicinal plants associated with the presence of a high number of bioactive compounds, namely phenolic compounds. The antioxidant, anti-inflammatory, and antimicrobial capacities of these molecules have aroused high interest in some industrial sectors, including food, pharmaceuticals, and cosmetics. This work aimed to determine the phenolic profiles of the infusions and hydroethanolic extracts of five plants (Carpobrotus edulis, Genista tridentata, Verbascum sinuatum, Cytisus multiflorus, and Calluna vulgaris) that have been employed in many traditional preparations. In addition, the antioxidant, antimicrobial, anti-inflammatory, and anti-tumoral activity of each different preparation was evaluated using in vitro assays. The HPLC-DAD-ESI/MS profile revealed the presence of eighty phenolic compounds, belonging to seven different families of compounds. Regarding antioxidant properties, the hydroethanolic extract of C. edulis showed a potent effect in the TBARS assay (IC50 = 1.20 µg/mL), while G. tridentata hydroethanolic extract achieved better results in the OxHLIA test (IC50 = 76 µg/mL). For cytotoxic and anti-inflammatory results, V. sinuatum infusions stood out significantly, with GI50 = 59.1-92.1 µg/mL and IC50 = 121.1 µg/mL, respectively. Finally, C. edulis hydroethanolic extract displayed the most relevant antibacterial activity, showing MBC values of 0.25-1 mg/mL, while G. tridentata hydroethanolic extract exerted the greatest antifungal effects (MFC of 0.5-1 mg/mL). The results of this study deepen the knowledge of the phenolic profiles and also provide evidence on the bioactive properties of the species selected, which could be considered highly valuable options for research and application in several sectors, namely food, cosmetics, and pharmaceuticals.

Pineapple by-products as a source of bioactive compounds with potential for industrial food application.

Pineapple is a tropical fruit consumed fresh or processed into various food products. However, the peel and crown of this fruit are not industrially exploited, thus generating tons of by-products that represent an economic and environmental concern. In order to promote the upcycling of these by-products, this work aimed to characterize the phenolic profile of its hydroethanolic extracts obtained from pineapple peel and crown leaves and to evaluate their in vitro bioactivity. The HPLC-DAD-ESI/MS analysis allowed the identification of 25 phenolic compounds, including phenolic acids and flavonoids. The antioxidant, cytotoxic, and antimicrobial activity assays highlighted the peel extract as the most promising and, therefore, it was incorporated into a traditional Portuguese pastry cake as a functional ingredient. The nutritional parameters of the developed food were not affected by the incorporation of the extract, but it promoted the antioxidant activity during its shelf-life. Overall, pineapple peel and crown appeared as promising by-products to be exploited by the food industry, which can be achieved through a circular economy approach.

The Effects of Probiotics, Prebiotics and Synbiotics in Non-Alcoholic Fat Liver Disease (NAFLD) and Non-Alcoholic Steatohepatitis (NASH): A Systematic Review.

Modifications in the microbiota caused by environmental and genetic reasons can unbalance the intestinal homeostasis, deregulating the host's metabolism and immune system, intensifying the risk factors for the development and aggravation of non-alcoholic fat liver disease (NAFLD). The use of probiotics, prebiotics and synbiotics have been considered a potential and promising strategy to regulate the gut microbiota and produce beneficial effects in patients with liver conditions. For this reason, this review aimed to evaluate the effectiveness of probiotics, prebiotics, and symbiotics in patients with NAFLD and NASH. Pubmed, Embase, and Cochrane databases were consulted, and PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analysis) guidelines were followed. The clinical trials used in this study demonstrated that gut microbiota interventions could improve a wide range of markers of inflammation, glycemia, insulin resistance, dyslipidemia, obesity, liver injury (decrease of hepatic enzymes and steatosis and fibrosis). Although microbiota modulators do not play a healing role, they can work as an important adjunct therapy in pathological processes involving NAFLD and its spectrums, either by improving the intestinal barrier or by preventing the formation of toxic metabolites for the liver or by acting on the immune system.

Application of Fibrin Associated with Photobiomodulation as a Promising Strategy to Improve Regeneration in Tissue Engineering: A Systematic Review.

Fibrin, derived from proteins involved in blood clotting (fibrinogen and thrombin), is a biopolymer with different applications in the health area since it has hemostasis, biocompatible and three-dimensional physical structure properties, and can be used as scaffolds in tissue regeneration or drug delivery system for cells and/or growth factors. Fibrin alone or together with other biomaterials, has been indicated for use as a biological support to promote the regeneration of stem cells, bone, peripheral nerves, and other injured tissues. In its diversity of forms of application and constitution, there are platelet-rich fibrin (PRF), Leukocyte- and platelet-rich fibrin (L-PRF), fibrin glue or fibrin sealant, and hydrogels. In order to increase fibrin properties, adjuvant therapies can be combined to favor tissue repair, such as photobiomodulation (PBM), by low-level laser therapy (LLLT) or LEDs (Light Emitting Diode). Therefore, this systematic review aimed to evaluate the relationship between PBM and the use of fibrin compounds, referring to the results of previous studies published in PubMed/MEDLINE, Scopus and Web of Science databases. The descriptors "fibrin AND low-level laser therapy" and "fibrin AND photobiomodulation" were used, without restriction on publication time. The bibliographic search found 44 articles in PubMed/MEDLINE, of which 26 were excluded due to duplicity or being outside the eligibility criteria. We also found 40 articles in Web of Science and selected 1 article, 152 articles in Scopus and no article selected, totaling 19 articles for qualitative analysis. The fibrin type most used in combination with PBM was fibrin sealant, mainly heterologous, followed by PRF or L-PRF. In PBM, the gallium-aluminum-arsenide (GaAlAs) laser prevailed, with a wavelength of 830 nm, followed by 810 nm. Among the preclinical studies, the most researched association of fibrin and PBM was the use of fibrin sealants in bone or nerve injuries; in clinical studies, the association of PBM with medication-related treatments osteonecrosis of the jaw (MRONJ). Therefore, there is scientific evidence of the contribution of PBM on fibrin composites, constituting a supporting therapy that acts by stimulating cell activity, angiogenesis, osteoblast activation, axonal growth, anti-inflammatory and anti-edema action, increased collagen synthesis and its maturation, as well as biomolecules.

A supervisão acadêmica do Projeto Mais Médicos para o Brasil na Bahia: facilidades, desafios e estratégias de intervenção pedagógica / Academic supervision within the Mais Médicos para o Brasil program in Bahia: facilities, challenges and strategies for pedagogic intervention / La supervisión académica del Proyecto Más Médicos para Brasil en Bahía: instalaciones, desafíos y estrategias en intervención pedagógica

Este artigo analisou as facilidades, dificuldades, ações e estratégias utilizadas na prática da supervisão acadêmica do Projeto Mais Médicos para o Brasil (PMMB) no estado da Bahia. Os dados foram produzidos por meio dos relatórios do sistema WebPortfólio pertencente à Universidade Aberta do Sistema Único de Saúde (UNA-SUS) e de entrevistas com os supervisores vinculados às instituições incluídas no programa. Os resultados demonstraram que a obrigatoriedade em ser supervisionado, o deslocamento para municípios distantes, a resistência dos médicos em relação à avaliação de suas condutas técnicas, as fragilidades da comunicação entre as coordenações estaduais e nacionais em relação à inabilidade técnica e o descumprimento de regras do programa são pontos que dificultam a supervisão acadêmica. A possibilidade de incluir as equipes na execução da supervisão da modalidade in loco foi considerada um facilitador. A supervisão longitudinal é favorecida por ser realizada coletivamente. Temas relacionados ao processo de trabalho, ao regramento do programa e relacionados às  atividades dos ciclos formativos eram mais recorrentes entre os médicos diplomados no Brasil. Quanto à realização de discussões clínicas e científicas sobre temas relevantes ao aperfeiçoamento das ações em Atenção Básica, percebe-se que eram priorizadas entre médicos cubanos, intercambistas individuais e médicos brasileiros diplomados no exterior. Este estudo trouxe a reflexão de que o contexto da pandemia pelo novo coronavírus trouxe impactos para a supervisão acadêmica. Além disso, a instabilidade política em torno do governo federal implicou uma descontinuidade na construção das diretrizes e dos espaços formativos, acarretando fragilidades na supervisão acadêmica.

This paper analyzes the facilities, difficulties, actions and strategies used by academic supervision within the Mais Médicos para o Brasil Program (PMMB) in the state of Bahia, Brazil. Data were collected by means of reports available in the WebPortfólio system, from the Open University of the Unified Health System (UNA-SUS), and interviews conducted with advisors linked to the institutions included in the program. Results show that the compulsory supervision, displacement to distant municipalities, the physicians' resistance towards an evaluation of their technical conducts, the communication gaps between the state and national coordinations regarding technical inability, and non-compliance with program rules are factors that hinder academic supervision. The possibility of including teams in on-site supervision was considered a facilitator. Longitudinal supervision is favored because it is carried out collectively. Brazilian physicians often look at themes related to the work process, program regulation and the activities developed in training cycles. In turn, clinical and scientific discussions on topics relevant for improving Primary Care actions were by Cuban doctors, individual exchange students and Brazilian doctors graduated abroad. This study demonstrates that the pandemic caused by the new coronavirus interfered with academic supervision. Moreover, the political instability surrounding the federal government generated discontinuities in the development of guidelines and training spaces, leading to gaps in academic supervision.

Este artículo analizó las facilidades, dificultades, acciones y estrategias utilizadas en la práctica de la supervisión académica del Proyecto Más Médicos para Brasil (PMMB) en el estado de Bahía. Los datos fueron recogidos de informes en el sistema WebPortfólio de la Universidad Aberta do Sistema Único de Saúde (UNA-SUS) y de entrevistas a supervisores vinculados a las instituciones incluidas en el programa. Los resultados demuestran que la obligación de ser supervisado; el desplazamiento hacia municipios lejanos; la resistencia de los médicos con relación a la evaluación de sus conductas técnicas; las debilidades con relación a la comunicación entre las coordinaciones estatales y nacionales respecto a la incapacidad técnica y el incumplimiento de las normas del programa son los puntos que dificultan la realización de la supervisión académica. Se consideró como facilitadora la posibilidad de incluir a los equipos en la ejecución de la supervisión en la modalidad in loco. Se favorece la supervisión longitudinal porque se lleva a cabo de forma colectiva. Los temas relacionados con el proceso de trabajo, las reglas del programa y las actividades de los ciclos de formación fueron los más recurrentes entre los médicos egresados de las facultades de medicina de Brasil. En cuanto a la realización de discusiones clínico-científicas sobre temas relevantes para el perfeccionamiento de las acciones en la Atención Primaria, se aprecia que fueron priorizadas entre médicos cubanos, estudiantes de intercambio y médicos brasileños graduados en el exterior. Este estudio plantea la reflexión de que el contexto de la pandemia provocada por el nuevo coronavirus trajo impactos a la supervisión académica. Además, la inestabilidad política en torno al gobierno federal implicó una discontinuidad en la construcción de lineamientos y espacios de formación, conllevando debilidades a la supervisión académica.

A atuação da supervisão acadêmica do Programa Mais Médicos no contexto da pandemia de Covid-19 na Bahia: um relato de experiência / Academic advising of the Mais Médicos Program during the Covid-19 pandemic in Bahia: an experience report / El desempeño de la supervisión académica del Programa Más Médicos en el contexto de la pandemia del Covid-19 en Bahía: un informe de experiencia

Este estudo tem o objetivo de relatar a experiência vivenciada pela equipe de apoio institucional do Ministério da Educação (MEC) no estado da Bahia em relação à supervisão acadêmica no contexto da pandemia de covid-19, no período de 2020 a 2021. Trata-se de uma pesquisa descritiva, do tipo relato de experiência, tendo como referência a atuação do apoio institucional frente à supervisão acadêmica no processo de trabalho dos médicos do Programa Mais Médicos para o Brasil (PMMB). Foram descritas as ações estratégicas e educacionais construídas pela supervisão acadêmica do PMMB no estado da Bahia, no período da pandemia pelo novo coronavírus, relacionadas às potencialidades e fragilidades encontradas no processo de trabalho nesse cenário. O contexto da pandemia impôs um grande desafio para os atores envolvidos no processo da supervisão acadêmica. Enfatiza-se, diante do cenário pandêmico, a importância da comunicação e articulação das instâncias do Sistema Único de Saúde (SUS) na organização do processo de trabalho nas unidades básicas de saúde e no enfrentamento da covid-19.

This study reports on the experience lived by the Institutional Support team from the Ministry of Education (MEC) in the state of Bahia, Brazil, concerning Academic Advising during the COVID-19 pandemic, from 2020 to 2021. This descriptive experience report concerns the institutional performance within Academic Advising in the work process of doctors from the Mais Médicos Program for Brazil (PMMB). It describes the strategic and educational actions built by the PMMB academic advising in Bahia during the pandemic caused by the new coronavirus, regarding the potentialities and weaknesses found in the work process in this context. The pandemic imposed a great challenge for the actors involved in the academic advising process. Given the pandemic scenario, the communication and articulation of instances from the Unified Health System (SUS) in organizing the work process in basic health units and in the fight against COVID-19 is of essence.

Este estudio describe la experiencia vivida por el equipo de apoyo institucional del Ministerio de Educación (MEC) en el estado de Bahía (Brasil) con relación a la supervisión académica en el contexto de la pandemia de Covid-19 en el período de 2020 a 2021. Se trata de un estudio descriptivo, de tipo informe de experiencia con referencia al desempeño institucional frente a la supervisión académica en el proceso de trabajo de los médicos del Programa Más Médicos para Brasil (PMM). Se describieron las acciones estratégicas y educativas construidas por la supervisión académica del PMMB en el estado de Bahía en el período de la pandemia provocada por el nuevo coronavirus, relacionadas con las fortalezas y debilidades encontradas en el proceso de trabajo en este contexto. El contexto de la pandemia ha impuesto un gran desafío para los actores involucrados en el proceso de supervisión académica. Ante este escenario, se destaca la importancia de la comunicación y articulación de las instancias del Sistema Único de Salud (SUS) en la organización del proceso de trabajo en las unidades básicas de salud y en el combate al Covid-19.